LabCalc

PCR Setup Calculator

V(component) = C(final) × V(reaction) / C(stock)

Per-reaction PCR component volumes from stock and final concentrations, scaled to a whole run with water to volume.

Reaction

µL

Master mix, enzyme, template — entered as a volume.

Components dosed by concentration

Result

0.528 mL pcr mix

24 reactions with 10% excess (factor 1.1)

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Calculation

  1. Scaling basis

    total = quantity per unit × number of units × (1 + excess / 100)

    24 reactions, 10% excess

    effective units = 26.4

  2. Fixed components (master mix, enzyme, buffer)

    11 µL × 24 reactions × 1.1 = 290.4 µL

    290.4 µL

  3. Forward primer

    0.8 µL × 24 reactions × 1.1 = 21.12 µL

    21.12 µL

  4. Reverse primer

    0.8 µL × 24 reactions × 1.1 = 21.12 µL

    21.12 µL

  5. dNTPs

    0.4 µL × 24 reactions × 1.1 = 10.56 µL

    10.56 µL

  6. Nuclease-free water (to volume)

    7 µL × 24 reactions × 1.1 = 184.8 µL

    184.8 µL

  7. Total pcr mix volume

    V(total) = Σ component volumes in the mix

    0.528 mL

  8. Volume per reaction

    20 µL

Inputs

Number of reactions
24
Excess
10 %
Fixed components (master mix, enzyme, buffer)
11 µL per reaction
Forward primer
0.8 µL per reaction
Reverse primer
0.8 µL per reaction
dNTPs
0.4 µL per reaction
Nuclease-free water (to volume)
7 µL per reaction

Totals include the excess factor you configured; the excess is deliberate over-preparation, not a measurement correction.

Components marked as added separately are excluded from the mixture total.

About this calculator

Each component volume is a straight C1V1 = C2V2 dilution into the reaction volume. Add the volume of any pre-mixed or fixed components (a 2× master mix, polymerase, template) and the calculator makes the reaction up to volume with water.

Stock and final concentrations must be the same kind of concentration — both molar or both mass-based. Converting between them needs a molecular weight, which is never assumed. The batch excess is applied only at the percentage you enter.

LabCalc is provided as a calculation aid. Users remain responsible for verifying calculations, units, chemical compatibility, experimental procedures and applicable safety requirements before laboratory use.

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