LabCalc

Master Mix Calculator

total = per unit × count × (1 + excess / 100)

Scale a per-reaction component list into a batch master mix, with an explicit excess allowance and every multiplication shown.

Mixture

Components (per reaction)

Result

0.5016 mL master mix

24 reactions with 10% excess (factor 1.1)

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Calculation

  1. Scaling basis

    total = quantity per unit × number of units × (1 + excess / 100)

    24 reactions, 10% excess

    effective units = 26.4

  2. 2× reaction buffer

    10 µL × 24 reactions × 1.1 = 264 µL

    264 µL

  3. Forward primer

    1 µL × 24 reactions × 1.1 = 26.4 µL

    26.4 µL

  4. Reverse primer

    1 µL × 24 reactions × 1.1 = 26.4 µL

    26.4 µL

  5. Nuclease-free water

    7 µL × 24 reactions × 1.1 = 184.8 µL

    184.8 µL

  6. Total master mix volume

    V(total) = Σ component volumes in the mix

    0.5016 mL

  7. Volume per reaction

    19 µL

Inputs

Number of reactions
24
Excess
10 %
2× reaction buffer
10 µL per reaction
Forward primer
1 µL per reaction
Reverse primer
1 µL per reaction
Nuclease-free water
7 µL per reaction

Totals include the excess factor you configured; the excess is deliberate over-preparation, not a measurement correction.

Components marked as added separately are excluded from the mixture total.

About this calculator

A master mix is prepared once and distributed across many reactions. Each component is scaled by the number of units you are preparing and by an excess allowance that covers pipetting losses and dead volume.

The excess is never applied silently: it defaults to the value you see and every component row shows its own multiplication. Components can be excluded from the mix total (for example template DNA added per tube) while still being scaled and reported.

LabCalc is provided as a calculation aid. Users remain responsible for verifying calculations, units, chemical compatibility, experimental procedures and applicable safety requirements before laboratory use.

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